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KMID : 1149220190270010053
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Korean Journal of Oriental Medical Prescription
2019 Volume.27 No. 1 p.53 ~ p.63
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Synergistic Anticancer Effect of the Cinnamomi Cortex Ethanol Extract (CcEE) and Hyperthermia in AGS Human Gastric Cancer Cells
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Park Sun-Hyang
Ahn Chae-Ryeong
Baek Seung-Ho
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Abstract
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Objectives : In this study, we investigated the combination effects of Cinnamomi cortex Ethanol Extract (CcEE) and hyperthermia in the human AGS gastric cancer cell line.
Methods : AGS cells were treated with the indicated concentrations of CcEE (0, 50 or 60 μg/mL) for 1h prior to hyperthermia. And then incubated for a further 30 min at the indicated temperatures (37, 42 or 43℃) in a humidified incubator containing 5% CO2 or a thermostatically controlled water bath for hyperthermia. The cell viability was measured by MTT assay, Morphology assay and Trypan blue assay. To investigate the possible molecular signaling pathways, the activation of mitogen-activated protein kinase (MAPK) proteins (ERK, p38 and JNK) and expression of various anti-apoptotic proteins such as Caspase-3, Caspase-9, p53, Cyclin D1 and MMP-2 were assessed by Western blot analysis. In addition, Annexin V and 7-amino-actinomycin D (7-AAD) staining was performed to examine the apoptotic mechanism.
Results : Combination of CcEE with hyperthermia effectively suppressed the cell viability and changed cellmorphology compared with CcEE or hyperthermia treatment alone. Combined treatment also abated the expression of Caspase-3, Caspase-9, Cyclin D1 and MMP-2. Whereas, the expression level of p53 was up-regulated by co-treatment. Moreover, combination treatment enhanced phosphorylation of ERK, p38 and JNK. In addition, this combination increased anti-cancer effect by inducing cell death through the apoptosis.
Conclusions : Taken together, all these findings suggest that the combination treatment with CcEE and hyperthermia may have therapeutic potential as a promising approach to patients with stomach cancer.
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KEYWORD
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Gastric cancer (GC), AGS, Cinnamomi cortex, Hyperthermia, Apoptosis, Combination, Synergistic effect, Anti-cancer effect, Mitogen-activated protein kinase (MAPK)
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